An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines - ScienceDirect
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning | Protocol
Cloning a lentiviral CRISPR construct
Viruses | Free Full-Text | Construction of a Recombinant Porcine Epidemic Diarrhea Virus Encoding Nanoluciferase for High-Throughput Screening of Natural Antiviral Products
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning | Protocol
Overhangs generated using BsmBI. | Download Scientific Diagram
James D. Thomas pgRNA cloning (updated: 06/12/2020) pgRNA cloning protocol used in Thomas et al (2020), Nature Genetics co
A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences | PLOS ONE
COA: FastDigest BsmBI (Esp3I), #FD0454
Addgene: p3E-DrCavin1a
CRISPR/Cas9 Cloning - Kay Hanggi
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries | ACS Synthetic Biology
Addgene: pENTR-L1-U6-BsmBI-R5
New England Biolabs, Inc. NEB Golden Gate Assembly Kit (BsmBI-v2) – 20 | Fisher Scientific
Thermo Scientific™ Esp3I (BsmBI) (10 U/µL) 10U/μL, 1000U Thermo Scientific™ Esp3I (BsmBI) (10 U/µL) | Fisher Scientific
Frontiers | Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors
FastDigest Esp3I (IIs class)
Schematic diagrams of TXO cell free system with minimal requirements.... | Download Scientific Diagram
Thermo Scientific™ Esp3I (BsmBI) (10 U/µL): Restriction Enzymes Enzymes and Inhibitors | Fisher Scientific
Petalsmiths Reading Group - HackMD
Problems of LentiCRISPR V2 digested with BsmBI
LaboShop | Products | Thermo Scientific™ Esp3I (BsmBI) (10 U/µL), 1,000 Units
Using a reverse genetics system to generate recombinant SARS-CoV-2 expressing robust levels of reporter genes | bioRxiv
Esp3I (BsmBI) (10 U/µL)
Thermo Scientific Molecular Biology Solutions 2012 - 2013 - Biocenter
